Leptin and ghrelin regulate dendritic cell maturation and dendritic cell induction of regulatory T-cells
Studied the influence of leptin, ghrelin, and thereof combinations in concentrations typical of pregnancy, to the maturation of dendritic cells and the induction of formation of regulatory T-lymphocytes. Installed that hormones do not affect the expression of molecules HLA-DR, CD83, CD86 on dendritic cells, reflecting their degree of maturity. The presence of leptin in the maturation process of dendritic cells enhances their production of IL-12, but does not affect the synthesis of TGF-1, IL-10, as well as the ability of dendritic cells to modulate the formation of Treg, Th17, and the production of IL-17A, IFN-, IL-4, IL-10 by co-cultivation with lymphocytes. Ghrelin, on the contrary, increases the production of TGF-1, and IL-10 by dendritic cells during their maturation, and does not affect on products of their IL-12 and induction of the formation of Treg and Th17, but inhibits the ability of dendritic cells to induce the production of IFN-, of IL-17A by co-cultivation with lymphocytes. The combination of hormones increased production of TGF-1 of dendritic cells and their ability to initiate the formation of Treg, reducing the production of IL-17A in co-culture with lymphocytes. Thus, leptin and ghrelin, modulating synthesis of polarizing cytokines by dendritic cells, determine the direction of differentiation of regulatory T-lymphocytes.
Key words: leptin, ghrelin, dendritic cells (DC), T-regulatory lymphocytes (Treg), IL-17-producing cells (Th17).